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vsmc cell line a10  (ATCC)


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    ATCC vsmc cell line a10
    NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The <t>VSMC</t> cell line, <t>A10,</t> was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.
    Vsmc Cell Line A10, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vsmc cell line a10/product/ATCC
    Average 97 stars, based on 1716 article reviews
    vsmc cell line a10 - by Bioz Stars, 2026-06
    97/100 stars

    Images

    1) Product Images from "Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation"

    Article Title: Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20092319

    NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The VSMC cell line, A10, was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.
    Figure Legend Snippet: NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The VSMC cell line, A10, was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.

    Techniques Used: Expressing, Staining, Microscopy, Immunofluorescence, Software

    A schematic diagram showing how NgBR is involved in MAM-regulated VSMC proliferation. Downregulation of NgBR disrupts the mitochondria-ER unit and increases MAM-associated pAkt-IP 3 R3 signaling, resulting in the suppression of mitochondrial function. Consequently, mitochondria-induced HIF-1α signaling is activated to stimulate cell proliferation.
    Figure Legend Snippet: A schematic diagram showing how NgBR is involved in MAM-regulated VSMC proliferation. Downregulation of NgBR disrupts the mitochondria-ER unit and increases MAM-associated pAkt-IP 3 R3 signaling, resulting in the suppression of mitochondrial function. Consequently, mitochondria-induced HIF-1α signaling is activated to stimulate cell proliferation.

    Techniques Used:



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    vsmc cell line a10  (ATCC)
    97
    ATCC vsmc cell line a10
    NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The <t>VSMC</t> cell line, <t>A10,</t> was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.
    Vsmc Cell Line A10, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vsmc cell line a10/product/ATCC
    Average 97 stars, based on 1 article reviews
    vsmc cell line a10 - by Bioz Stars, 2026-06
    97/100 stars
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    rat vascular smooth muscle cell vsmc line a10  (ATCC)
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    ATCC rat vascular smooth muscle cell vsmc line a10
    NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The <t>VSMC</t> cell line, <t>A10,</t> was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.
    Rat Vascular Smooth Muscle Cell Vsmc Line A10, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat vascular smooth muscle cell vsmc line a10/product/ATCC
    Average 97 stars, based on 1 article reviews
    rat vascular smooth muscle cell vsmc line a10 - by Bioz Stars, 2026-06
    97/100 stars
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    vsmc a10 cell line  (ATCC)
    97
    ATCC vsmc a10 cell line
    NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The <t>VSMC</t> cell line, <t>A10,</t> was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.
    Vsmc A10 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vsmc a10 cell line/product/ATCC
    Average 97 stars, based on 1 article reviews
    vsmc a10 cell line - by Bioz Stars, 2026-06
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The VSMC cell line, A10, was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation

    doi: 10.3390/ijms20092319

    Figure Lengend Snippet: NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia. Rats were housed in hypobaric hypoxia (10.0% O 2 ) for 45 days, and then exposed to reoxygenation at different times. Pulmonary vascular remodeling was evaluated by measuring the ( A ) mPAP and ( B ) weight ratio of RV/ (LV + S). ( C , D ) Histological images of distal pulmonary arteries (arrows)stained with hematoxylin and eosin. Scale bar = 100 µm. The percent medial wall thickness was analyzed using an Olympus IX-70 microscope. n = 44–50 pulmonary arteries (35–100 µm) from three animals/group. ( E ) The small pulmonary arteries (30–140 µm) were co-stained with α-smooth muscle actin (α-SMA; green) and NgBR (red). Nuclei were stained with DAPI (blue). Results were calculated as a relative arbitrary immunofluorescence unit (AFU) using FV10-ASW3.1 software. n = 38–54 pulmonary arteries from three animals/group. Scale bar = 30 µm. The VSMC cell line, A10, was exposed to 4% O 2 for 48 h, followed by exposure to 21% O 2 for 24 h. ( F , G ) Cell proliferation was evaluated by determining PCNA expression. n = 4. Apoptosis was detected by analyzing cleaved-capase-3 expression. n = 3. ** p < 0.01 H48 versus N48, R24 versus N72. ( H ) NgBR expression was normalized to β-actin. n = 3. * p < 0.05.

    Article Snippet: The VSMC cell line A10 was purchased from the American Type Culture Collection and cultured in high-glucose Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

    Techniques: Expressing, Staining, Microscopy, Immunofluorescence, Software

    A schematic diagram showing how NgBR is involved in MAM-regulated VSMC proliferation. Downregulation of NgBR disrupts the mitochondria-ER unit and increases MAM-associated pAkt-IP 3 R3 signaling, resulting in the suppression of mitochondrial function. Consequently, mitochondria-induced HIF-1α signaling is activated to stimulate cell proliferation.

    Journal: International Journal of Molecular Sciences

    Article Title: Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation

    doi: 10.3390/ijms20092319

    Figure Lengend Snippet: A schematic diagram showing how NgBR is involved in MAM-regulated VSMC proliferation. Downregulation of NgBR disrupts the mitochondria-ER unit and increases MAM-associated pAkt-IP 3 R3 signaling, resulting in the suppression of mitochondrial function. Consequently, mitochondria-induced HIF-1α signaling is activated to stimulate cell proliferation.

    Article Snippet: The VSMC cell line A10 was purchased from the American Type Culture Collection and cultured in high-glucose Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

    Techniques: